FastQCFastQC Report
Wed 5 Jul 2017
FCHGVKNBBXX-HKZEBggcRAAHRAAPEI-210_L3_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameFCHGVKNBBXX-HKZEBggcRAAHRAAPEI-210_L3_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37737419
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCG18250.016.5133343
CGATCGA16700.016.3337864
CCGCGTA26700.015.98372127
CGGAATC36150.014.4894852
CGTACTA29200.014.46513535
TCGCGTA12400.014.3698228
CCGAGCG111900.013.85966638
CGAGCGC114650.013.43116639
GCGCACC118000.013.180642
ACTCGAC36200.013.1258325
CCGCCTA44850.012.99758916
CTACCGA22600.012.84834423
CGCACCT120250.012.82463843
CGCGTAC33850.012.6075628
GTACCGG83650.012.5971935
AACTACG55200.012.59301410
GTCGCGT14200.012.54827627
CTCAGAT173050.012.4588181
CAATCGA29300.012.3879094
ACCGAGC126650.012.332396537
Produced by FastQC (version 0.11.5)